TY - JOUR

T1 - Analysis of six DNA components of the faba bean necrotic yellows virus genome and their structural affinity to related plant virus genomes

AU - Katul, Lina

AU - Maiss, Edgar

AU - Morozov, Sergey Yu

AU - Vetten, H. Josef

N1 - Funding Information: We thank Uli Commandeur and Phil Berger for their assistance with sequence analysis, Bruno Gronenborn for critical comments on the manuscript, and James L. Dale for sharing information prior to publication. This work was supported by a grant of the Bundesministerium für Wirtschaftliche Zusammenarbeit (BMZ).

PY - 2002/5/25

Y1 - 2002/5/25

N2 - Faba bean necrotic yellows virus (FBNYV) has a multicomponent circular ssDNA genome. In addition to a previously described genome component (C1) coding for a replicase-associated protein (Rep), five further components (C2 to C6) have now been identified. Each of the six components is about 1 kb in size, contains one major open reading frame (ORF) in the virion sense with a TATA box and polyadenylation signal, and has a noncoding region containing a highly conserved sequence possibly forming s stem-loop structure. Similar to C1, C2 encodes another putative Rep of 33.1 kDa, which is closely related to the Rap of banana bunchy top virus (BBTV). Based on bacterial expression and immunoblot analysis, the ORF of C5 encodes the capsid protein (CP) with a deduced molecular mass of 19 kDa. The FBNYV CP shares the highest amino acid (aa) identity (56.2%) with that of subterranean clover stunt virus (SCSV]. The ORF of C4 potentially codes for a hydrophobic protein which appears to be structurally and functionally similar to the BBTV-C4 and SCSV-C1 proteins. No protein sequence similarities were found in databases for the C3 and C6 ORFs of FBNYV. FBNYV is clearly distinct from any known virus but is taxonomically related to BBTV and SCSV.

AB - Faba bean necrotic yellows virus (FBNYV) has a multicomponent circular ssDNA genome. In addition to a previously described genome component (C1) coding for a replicase-associated protein (Rep), five further components (C2 to C6) have now been identified. Each of the six components is about 1 kb in size, contains one major open reading frame (ORF) in the virion sense with a TATA box and polyadenylation signal, and has a noncoding region containing a highly conserved sequence possibly forming s stem-loop structure. Similar to C1, C2 encodes another putative Rep of 33.1 kDa, which is closely related to the Rap of banana bunchy top virus (BBTV). Based on bacterial expression and immunoblot analysis, the ORF of C5 encodes the capsid protein (CP) with a deduced molecular mass of 19 kDa. The FBNYV CP shares the highest amino acid (aa) identity (56.2%) with that of subterranean clover stunt virus (SCSV]. The ORF of C4 potentially codes for a hydrophobic protein which appears to be structurally and functionally similar to the BBTV-C4 and SCSV-C1 proteins. No protein sequence similarities were found in databases for the C3 and C6 ORFs of FBNYV. FBNYV is clearly distinct from any known virus but is taxonomically related to BBTV and SCSV.

UR - http://www.scopus.com/inward/record.url?scp=0030803435&partnerID=8YFLogxK

U2 - 10.1006/viro.1997.8611

DO - 10.1006/viro.1997.8611

M3 - Article

C2 - 9217049

AN - SCOPUS:0030803435

VL - 233

SP - 247

EP - 259

JO - VIROLOGY

JF - VIROLOGY

SN - 0042-6822

IS - 2

ER -