TY - JOUR

T1 - Analysis of poration-induced changes in cells from laser-activated plasmonic substrates

AU - Saklayen, Nabiha

AU - Kalies, Stefan

AU - Madrid, Marinna

AU - Nuzzo, Valeria

AU - Huber, Marinus

AU - Shen, Weilu

AU - Sinanan-Singh, Jasmine

AU - Heinemann, Dag

AU - Heisterkamp, Alexander

AU - Mazur, Eric

N1 - Funding information: NS, MM, WS, JSS, and EM were supported by the National Science Foundation under contracts PHY-1219334 and PHY-1205465. NS was funded by the Howard Hughes Medical Institute’s International Fellowship and the short-term research fellowship from DAAD. MM was funded by the Graduate Prize Fellowship at Harvard University. SK and AH received funding from the German Research Foundation through the Cluster of Excellence REBIRTH (DFG EXC62/3). Parts of this work were carried out as an integral part of the BIOFABRICATION FOR NIFE Initiative, which is financially supported by the ministry of Lower Saxony and the Volkswagen Stiftung (BIOFABRICATION FOR NIFE: VWZN2860). NN, MM, and EM have an interest in Cellino Biotech.

PY - 2017/10

Y1 - 2017/10

N2 - Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 - 10% decrease), and cytoplasm (5 - 10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications.

AB - Laser-exposed plasmonic substrates permeabilize the plasma membrane of cells when in close contact to deliver cell-impermeable cargo. While studies have determined the cargo delivery efficiency and viability of laser-exposed plasmonic substrates, morphological changes in a cell have not been quantified. We porated myoblast C2C12 cells on a plasmonic pyramid array using a 532-nm laser with 850-ps pulse length and time-lapse fluorescence imaging to quantify cellular changes. We obtain a poration efficiency of 80%, viability of 90%, and a pore radius of 20 nm. We quantified area changes in the plasma membrane attached to the substrate (10% decrease), nucleus (5 - 10% decrease), and cytoplasm (5 - 10% decrease) over 1 h after laser treatment. Cytoskeleton fibers show a change of 50% in the alignment, or coherency, of fibers, which stabilizes after 10 mins. We investigate structural and morphological changes due to the poration process to enable the safe development of this technique for therapeutic applications.

KW - (000.1430) biology and medicine

KW - (190.4870) photothermal effects

KW - (350.4855) optical tweezers or optical manipulation

UR - http://www.scopus.com/inward/record.url?scp=85031022540&partnerID=8YFLogxK

U2 - 10.1364/BOE.8.004756

DO - 10.1364/BOE.8.004756

M3 - Article

AN - SCOPUS:85031022540

VL - 8

SP - 4756

EP - 4771

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 10

M1 - #303310

ER -