TY - JOUR

T1 - Analysis of cell wall proteins regulated in stem of susceptible and resistant tomato species after inoculation with Ralstonia solanacearum

T2 - A proteomic approach

AU - Dahal, Diwakar

AU - Pich, Andreas

AU - Braun, Hans Peter

AU - Wydra, Kerstin

N1 - Funding information: Acknowledgments This study was funded by the Federal Ministry for Economic Cooperation and Development (BMZ), Germany, in the framework of the collaborative project number 03.7860.4–001.00 with the Asian Vegetable Research and Development Center (AVRDC), Taiwan.

PY - 2010/5/23

Y1 - 2010/5/23

N2 - Proteomics approach was used to elucidate the molecular interactions taking place at the stem cell wall level when tomato species were inoculated with Ralstonia solanacearum, a causative agent of bacterial wilt. Cell wall proteins from both resistant and susceptible plants before and after the bacterial inoculation were extracted from purified cell wall with salt buffers and separated with 2-D IEF/SDS-PAGE and with 3-D IEF/SDS/SDS-PAGE for basic proteins. The gels stained with colloidal Coomassie revealed varied abundance of protein spots between two species (eight proteins in higher abundance in resistant and six other in susceptible). Moreover, proteins were regulated differentially in response to bacterial inoculation in resistant (seven proteins increased and eight other decreased) as well as in susceptible plants (five proteins elevated and eight other suppressed). Combination of MALDI-TOF/TOF MS and LC-ESI-IonTrap MS/MS lead to the identification of those proteins. Plants responded to pathogen inoculation by elevating the expression of pathogenesis related, other defense related and glycolytic proteins in both species. However, cell wall metabolic proteins in susceptible, and antioxidant, stress related as well as energy metabolism proteins in resistant lines were suppressed. Most of the proteins of the comparative analysis and other randomly picked spots were predicted to have secretion signals except some classical cytosolic proteins.

AB - Proteomics approach was used to elucidate the molecular interactions taking place at the stem cell wall level when tomato species were inoculated with Ralstonia solanacearum, a causative agent of bacterial wilt. Cell wall proteins from both resistant and susceptible plants before and after the bacterial inoculation were extracted from purified cell wall with salt buffers and separated with 2-D IEF/SDS-PAGE and with 3-D IEF/SDS/SDS-PAGE for basic proteins. The gels stained with colloidal Coomassie revealed varied abundance of protein spots between two species (eight proteins in higher abundance in resistant and six other in susceptible). Moreover, proteins were regulated differentially in response to bacterial inoculation in resistant (seven proteins increased and eight other decreased) as well as in susceptible plants (five proteins elevated and eight other suppressed). Combination of MALDI-TOF/TOF MS and LC-ESI-IonTrap MS/MS lead to the identification of those proteins. Plants responded to pathogen inoculation by elevating the expression of pathogenesis related, other defense related and glycolytic proteins in both species. However, cell wall metabolic proteins in susceptible, and antioxidant, stress related as well as energy metabolism proteins in resistant lines were suppressed. Most of the proteins of the comparative analysis and other randomly picked spots were predicted to have secretion signals except some classical cytosolic proteins.

KW - 3-D PAGE

KW - Defense and metabolic proteins

KW - R. solanacearum

KW - Secretory proteins

KW - Stem cell wall proteome

KW - Tomato species

UR - http://www.scopus.com/inward/record.url?scp=77954425022&partnerID=8YFLogxK

U2 - 10.1007/s11103-010-9646-z

DO - 10.1007/s11103-010-9646-z

M3 - Article

C2 - 20496099

AN - SCOPUS:77954425022

VL - 73

SP - 643

EP - 658

JO - Plant molecular biology

JF - Plant molecular biology

SN - 0167-4412

IS - 6

ER -