Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 844-871 |
| Seitenumfang | 28 |
| Fachzeitschrift | Metabolites |
| Jahrgang | 2 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 12 Nov. 2012 |
Abstract
Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Endokrinologie, Diabetes und Stoffwechsel
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
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in: Metabolites, Jahrgang 2, Nr. 4, 12.11.2012, S. 844-871.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Analysis and design of stimulus response curves of E. coli
AU - Kremling, Andreas
AU - Goehler, Anna
AU - Jahreis, Knut
AU - Nees, Markus
AU - Auerbach, Benedikt
AU - Schmidt-Heck, Wolfgang
AU - Kökpinar, Öznur
AU - Geffers, Robert
AU - Rinas, Ursula
AU - Bettenbrock, Katja
PY - 2012/11/12
Y1 - 2012/11/12
N2 - Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
AB - Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
KW - Carbohydrate uptake
KW - Escherichia coli
KW - Feedforward control
KW - Gene regulatory network
KW - Network component analysis
KW - Parameter estimation
UR - http://www.scopus.com/inward/record.url?scp=85043430540&partnerID=8YFLogxK
U2 - 10.3390/metabo2040844
DO - 10.3390/metabo2040844
M3 - Article
AN - SCOPUS:85043430540
VL - 2
SP - 844
EP - 871
JO - Metabolites
JF - Metabolites
IS - 4
ER -