An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Externe Organisationen

  • Rheinische Friedrich-Wilhelms-Universität Bonn
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Details

OriginalspracheEnglisch
Seiten (von - bis)157-160
Seitenumfang4
FachzeitschriftAnalytical biochemistry
Jahrgang176
Ausgabenummer1
PublikationsstatusVeröffentlicht - Jan. 1989
Extern publiziertJa

Abstract

A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biophysik
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Biochemie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Molekularbiologie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Zellbiologie

Zitieren

An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays. / Reinard, Thomas; Jacobsen, Hans Jörg.
in: Analytical biochemistry, Jahrgang 176, Nr. 1, 01.1989, S. 157-160.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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