Details
Originalsprache | Englisch |
---|---|
Seiten (von - bis) | 157-160 |
Seitenumfang | 4 |
Fachzeitschrift | Analytical biochemistry |
Jahrgang | 176 |
Ausgabenummer | 1 |
Publikationsstatus | Veröffentlicht - Jan. 1989 |
Extern publiziert | Ja |
Abstract
A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biophysik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Zellbiologie
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in: Analytical biochemistry, Jahrgang 176, Nr. 1, 01.1989, S. 157-160.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - An Inexpensive Small Volume Equilibrium Dialysis System for Protein-Ligand Binding Assays
AU - Reinard, Thomas
AU - Jacobsen, Hans Jörg
PY - 1989/1
Y1 - 1989/1
N2 - A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
AB - A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.
UR - http://www.scopus.com/inward/record.url?scp=0024551980&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(89)90286-8
DO - 10.1016/0003-2697(89)90286-8
M3 - Article
C2 - 2712285
AN - SCOPUS:0024551980
VL - 176
SP - 157
EP - 160
JO - Analytical biochemistry
JF - Analytical biochemistry
SN - 0003-2697
IS - 1
ER -