An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Valerie Beneke
  • Fennja Küster
  • Anna Lena Neehus
  • Christina Hesse
  • Elena Lopez-Rodriguez
  • Kathrin Haake
  • Anna Rafiei Hashtchin
  • Juliane Wilhelmine Schott
  • Dorothee Walter
  • Armin Braun
  • Willem F. Wolkers
  • Mania Ackermann
  • Nico Lachmann

Organisationseinheiten

Externe Organisationen

  • Medizinische Hochschule Hannover (MHH)
  • Fraunhofer-Institut für Toxikologie und Experimentelle Medizin (ITEM)
  • REBIRTH Forschungszentrum für translationale regenerative Medizin
  • Deutsches Zentrum für Lungenforschung (DZL)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer16281
FachzeitschriftScientific reports
Jahrgang8
PublikationsstatusVeröffentlicht - 2 Nov. 2018

Abstract

Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform.

ASJC Scopus Sachgebiete

Zitieren

An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. / Beneke, Valerie; Küster, Fennja; Neehus, Anna Lena et al.
in: Scientific reports, Jahrgang 8, 16281, 02.11.2018.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Beneke, V, Küster, F, Neehus, AL, Hesse, C, Lopez-Rodriguez, E, Haake, K, Rafiei Hashtchin, A, Schott, JW, Walter, D, Braun, A, Wolkers, WF, Ackermann, M & Lachmann, N 2018, 'An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages', Scientific reports, Jg. 8, 16281. https://doi.org/10.1038/s41598-018-34524-2, https://doi.org/10.15488/4728
Beneke, V., Küster, F., Neehus, A. L., Hesse, C., Lopez-Rodriguez, E., Haake, K., Rafiei Hashtchin, A., Schott, J. W., Walter, D., Braun, A., Wolkers, W. F., Ackermann, M., & Lachmann, N. (2018). An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. Scientific reports, 8, Artikel 16281. https://doi.org/10.1038/s41598-018-34524-2, https://doi.org/10.15488/4728
Beneke V, Küster F, Neehus AL, Hesse C, Lopez-Rodriguez E, Haake K et al. An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. Scientific reports. 2018 Nov 2;8:16281. doi: 10.1038/s41598-018-34524-2, 10.15488/4728
Beneke, Valerie ; Küster, Fennja ; Neehus, Anna Lena et al. / An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. in: Scientific reports. 2018 ; Jahrgang 8.
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title = "An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages",
abstract = "Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform.",
author = "Valerie Beneke and Fennja K{\"u}ster and Neehus, {Anna Lena} and Christina Hesse and Elena Lopez-Rodriguez and Kathrin Haake and {Rafiei Hashtchin}, Anna and Schott, {Juliane Wilhelmine} and Dorothee Walter and Armin Braun and Wolkers, {Willem F.} and Mania Ackermann and Nico Lachmann",
note = "Funding Information: The authors thank Doreen L{\"u}ttge and Theresa Buchegger (Hannover Medical School) for excellent technical assistance. Moreover, we would like to thank Prof. Thomas Moritz for critical comments and his support. The authors also thank Daniela Paasch for providing genetically transduced human U937 cells. This work was financially supported by grants from the Deutsche Forschungsgemeinschaft: Cluster of Excellence REBIRTH (Exc 62/2) and the grant LA3680/2-1 (N.L.). The work was also funded by the Helmholtz excellence network REBIRTHt4s. Moreover, the work was supported by grants from the Federal Ministry of Education and Research (BMBF iMACnet 01EK1602A), the Else Kr{\"o}ner-Fresenius-Stiftung (EKFS): 2015_92 (N.L.) and 2016_A146 (M.A.). This work was also supported by a scholarship of the REBIRTH PhD program Regenerative Sciences (K.H.). ",
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T1 - An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages

AU - Beneke, Valerie

AU - Küster, Fennja

AU - Neehus, Anna Lena

AU - Hesse, Christina

AU - Lopez-Rodriguez, Elena

AU - Haake, Kathrin

AU - Rafiei Hashtchin, Anna

AU - Schott, Juliane Wilhelmine

AU - Walter, Dorothee

AU - Braun, Armin

AU - Wolkers, Willem F.

AU - Ackermann, Mania

AU - Lachmann, Nico

N1 - Funding Information: The authors thank Doreen Lüttge and Theresa Buchegger (Hannover Medical School) for excellent technical assistance. Moreover, we would like to thank Prof. Thomas Moritz for critical comments and his support. The authors also thank Daniela Paasch for providing genetically transduced human U937 cells. This work was financially supported by grants from the Deutsche Forschungsgemeinschaft: Cluster of Excellence REBIRTH (Exc 62/2) and the grant LA3680/2-1 (N.L.). The work was also funded by the Helmholtz excellence network REBIRTHt4s. Moreover, the work was supported by grants from the Federal Ministry of Education and Research (BMBF iMACnet 01EK1602A), the Else Kröner-Fresenius-Stiftung (EKFS): 2015_92 (N.L.) and 2016_A146 (M.A.). This work was also supported by a scholarship of the REBIRTH PhD program Regenerative Sciences (K.H.).

PY - 2018/11/2

Y1 - 2018/11/2

N2 - Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform.

AB - Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform.

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