Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 391-7 |
| Seitenumfang | 7 |
| Fachzeitschrift | Clinical Hemorheology and Microcirculation |
| Jahrgang | 49 |
| Ausgabenummer | 1-4 |
| Publikationsstatus | Veröffentlicht - 2011 |
Abstract
Endothelial cells (ECs) are permanently exposed to the blood flow and the resulting shear stress, its magnitude varying with the EC site in the blood stream. Along with other mechanical stimuli like vessel wall stretching or hydrostatic blood pressure, this shear stress modulates the endothelial cell function, morphology and gene expression. Here, we describe our improved cone-and-plate reactor that applies up to 10 dyn/cm(2) uniform wall shear stress on a defined, ring-shaped region on a culture dish. At the same time, a hydrostatic pressure of up to 195 mmHg can be applied by increasing the atmospheric pressure in the incubator box. Gas composition can be controlled additionally, used for maintaining CO2-homeostasis or inducing hypoxic conditions. For better comparability, six cone-and-plate systems can be used at the same time at different rotational velocities. The effects on cell morphology, cytoskeleton and cell alignment can be monitored during application using a laser scanning microscope. Flow conditions have been studied and a sufficient area of uniform wall shear stress could be shown. To exceed 10 dyn/cm2, we suggest an increase in medium viscosity.
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in: Clinical Hemorheology and Microcirculation, Jahrgang 49, Nr. 1-4, 2011, S. 391-7.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - An advanced cone-and-plate reactor for the in vitro-application of shear stress on adherent cells
AU - Dreyer, Lutz
AU - Krolitzki, Benjamin
AU - Autschbach, Rüdiger
AU - Vogt, Peter
AU - Welte, Tobias
AU - Ngezahayo, Anaclet
AU - Glasmacher, Birgit
PY - 2011
Y1 - 2011
N2 - Endothelial cells (ECs) are permanently exposed to the blood flow and the resulting shear stress, its magnitude varying with the EC site in the blood stream. Along with other mechanical stimuli like vessel wall stretching or hydrostatic blood pressure, this shear stress modulates the endothelial cell function, morphology and gene expression. Here, we describe our improved cone-and-plate reactor that applies up to 10 dyn/cm(2) uniform wall shear stress on a defined, ring-shaped region on a culture dish. At the same time, a hydrostatic pressure of up to 195 mmHg can be applied by increasing the atmospheric pressure in the incubator box. Gas composition can be controlled additionally, used for maintaining CO2-homeostasis or inducing hypoxic conditions. For better comparability, six cone-and-plate systems can be used at the same time at different rotational velocities. The effects on cell morphology, cytoskeleton and cell alignment can be monitored during application using a laser scanning microscope. Flow conditions have been studied and a sufficient area of uniform wall shear stress could be shown. To exceed 10 dyn/cm2, we suggest an increase in medium viscosity.
AB - Endothelial cells (ECs) are permanently exposed to the blood flow and the resulting shear stress, its magnitude varying with the EC site in the blood stream. Along with other mechanical stimuli like vessel wall stretching or hydrostatic blood pressure, this shear stress modulates the endothelial cell function, morphology and gene expression. Here, we describe our improved cone-and-plate reactor that applies up to 10 dyn/cm(2) uniform wall shear stress on a defined, ring-shaped region on a culture dish. At the same time, a hydrostatic pressure of up to 195 mmHg can be applied by increasing the atmospheric pressure in the incubator box. Gas composition can be controlled additionally, used for maintaining CO2-homeostasis or inducing hypoxic conditions. For better comparability, six cone-and-plate systems can be used at the same time at different rotational velocities. The effects on cell morphology, cytoskeleton and cell alignment can be monitored during application using a laser scanning microscope. Flow conditions have been studied and a sufficient area of uniform wall shear stress could be shown. To exceed 10 dyn/cm2, we suggest an increase in medium viscosity.
KW - Bioreactors
KW - Cells, Cultured/cytology
KW - Culture Media
KW - Endothelial Cells/cytology
KW - Endothelium, Vascular/cytology
KW - Environment, Controlled
KW - Equipment Design
KW - Hemorheology
KW - Humans
KW - Hydrostatic Pressure
KW - Microscopy, Confocal/instrumentation
KW - Rheology/instrumentation
KW - Rotation
KW - Shear Strength
KW - Stress, Mechanical
KW - Viscosity
U2 - 10.3233/CH-2011-1488
DO - 10.3233/CH-2011-1488
M3 - Article
C2 - 22214709
VL - 49
SP - 391
EP - 397
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
SN - 1386-0291
IS - 1-4
ER -