TY - JOUR

T1 - All-Optical Planar Polymer Waveguide-Based Biosensor Chip Designed for Smartphone-Assisted Detection of Vitamin D

AU - Walter, Johanna-Gabriela

AU - Alwis, Lourdes S.M.

AU - Roth, Bernhard

AU - Bremer, Kort

N1 - Funding Information: Funding: B.R. and K.B. acknowledges funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). J.-G.W. and K.B. acknowledge support from the Bundesministerium für Wirtschaft und Energie (BMWi) as well as the European Social Fund (ESF) within Grant Number 03EFHNI059.

PY - 2020/11/27

Y1 - 2020/11/27

N2 - An all-optical plasmonic sensor platform designed for smartphones based on planar-optical waveguide structures integrated in a polymer chip is reported for the first time. To demonstrate the applicability of the sensor system for biosensing purposes, the detection of 25-hydroxyvitamin D (25OHD) in human serum samples using an AuNP-enhanced aptamer-based assay was demonstrated. With the aid of the developed assay sensitivity of 0.752 pixel/nM was achieved for 25OHD concentrations ranging from 0–100 nM. The waveguide structure of the sensor enables miniaturisation and parallelisation, thus, demonstrates the potential for simultaneous detection of various analytes including biomarkers. The entire optical arrangement can be integrated into a single polymer chip which allows for large scale and cost-efficient sensor fabrication. The broad utilization and access of smartphone electronics make the proposed design most attractive for its wider use in lab-on-chip applications.

AB - An all-optical plasmonic sensor platform designed for smartphones based on planar-optical waveguide structures integrated in a polymer chip is reported for the first time. To demonstrate the applicability of the sensor system for biosensing purposes, the detection of 25-hydroxyvitamin D (25OHD) in human serum samples using an AuNP-enhanced aptamer-based assay was demonstrated. With the aid of the developed assay sensitivity of 0.752 pixel/nM was achieved for 25OHD concentrations ranging from 0–100 nM. The waveguide structure of the sensor enables miniaturisation and parallelisation, thus, demonstrates the potential for simultaneous detection of various analytes including biomarkers. The entire optical arrangement can be integrated into a single polymer chip which allows for large scale and cost-efficient sensor fabrication. The broad utilization and access of smartphone electronics make the proposed design most attractive for its wider use in lab-on-chip applications.

KW - Aptamers

KW - Biosensor

KW - Integrated optics

KW - Optical waveguide

KW - Surface plasmon resonance

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=85096720202&partnerID=8YFLogxK

U2 - 10.3390/s20236771

DO - 10.3390/s20236771

M3 - Article

AN - SCOPUS:85096720202

VL - 20

JO - Sensors (Switzerland)

JF - Sensors (Switzerland)

SN - 1424-8220

IS - 23

M1 - 6771

ER -