Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: Factors affecting transformation and regeneration of transgenic shoots

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Sridevy Sriskandarajah
  • Stefan Frello
  • Kirsten Jørgensen
  • Margrethe Serek

Externe Organisationen

  • Københavns Universitet
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)59-63
Seitenumfang5
FachzeitschriftPlant cell reports
Jahrgang23
Ausgabenummer1-2
PublikationsstatusVeröffentlicht - 28 Apr. 2004

Abstract

An efficient transformation system for Campanula carpatica was developed using Agrobacterium tumefaciens strains LBA4404 (harbouring the plasmid pBI121), and AGLO (harbouring the plasmid pBEO210). This is the first report on the transformation of C. carpatica. Various factors affecting the transformation efficiency and subsequent regeneration were identified. The age of seedlings from which the explants for transformation studies were taken, and the growth conditions under which the seedlings were grown had a significant influence on the production of transformed shoots. Hypocotyls taken from 12-day-old seedlings grown in the dark were the most productive, with up to 25% of hypocotyls producing transformed shoots. Explants taken from 5-week-old seedlings produced only transformed callus. The medium used for co-cultivation and incubation also had a significant influence on transformation frequency and shoot regeneration. The cultivar "Blue Uniform" was more responsive than "White Uniform". Both bacterial strains and plasmids were equally effective in producing transformed tissue. Transformed shoots were selected on kanamycin medium, and the presence of the uidA and nptII genes in those selected shoots was confirmed by β-glucuronidase and ELISA analyses, respectively.

ASJC Scopus Sachgebiete

Zitieren

Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: Factors affecting transformation and regeneration of transgenic shoots. / Sriskandarajah, Sridevy; Frello, Stefan; Jørgensen, Kirsten et al.
in: Plant cell reports, Jahrgang 23, Nr. 1-2, 28.04.2004, S. 59-63.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Sriskandarajah S, Frello S, Jørgensen K, Serek M. Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: Factors affecting transformation and regeneration of transgenic shoots. Plant cell reports. 2004 Apr 28;23(1-2):59-63. doi: 10.1007/s00299-004-0797-8
Sriskandarajah, Sridevy ; Frello, Stefan ; Jørgensen, Kirsten et al. / Agrobacterium tumefaciens-mediated transformation of Campanula carpatica : Factors affecting transformation and regeneration of transgenic shoots. in: Plant cell reports. 2004 ; Jahrgang 23, Nr. 1-2. S. 59-63.
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