Agricultural soil and drilosphere as reservoirs of new and unusual assimilators of 2,4-dichlorophenol carbon

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OriginalspracheEnglisch
Seiten (von - bis)84-100
Seitenumfang17
FachzeitschriftEnvironmental microbiology
Jahrgang16
Ausgabenummer1
PublikationsstatusVeröffentlicht - Jan. 2014

Abstract

2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.

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Agricultural soil and drilosphere as reservoirs of new and unusual assimilators of 2,4-dichlorophenol carbon. / Dallinger, Anja; Horn, Marcus A.
in: Environmental microbiology, Jahrgang 16, Nr. 1, 01.2014, S. 84-100.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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title = "Agricultural soil and drilosphere as reservoirs of new and unusual assimilators of 2,4-dichlorophenol carbon",
abstract = "2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.",
author = "Anja Dallinger and Horn, {Marcus A.}",
note = "Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "2014",
month = jan,
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volume = "16",
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T1 - Agricultural soil and drilosphere as reservoirs of new and unusual assimilators of 2,4-dichlorophenol carbon

AU - Dallinger, Anja

AU - Horn, Marcus A.

N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 2014/1

Y1 - 2014/1

N2 - 2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.

AB - 2,4-Dichlorophenol (2,4-DCP) is a potential soil and groundwater contaminant. Earthworms modulate growth and activities of soil microbiota. Thus, active 2,4-DCP degraders in agricultural soil and drilosphere (i.e. burrow walls, gut content and cast) were identified by comparative amplicon pyrosequencing-based 16S rRNA stable isotope probing in soil columns. In situ relevant concentrations of [U- 13C]2,4-DCP were consumed in soil within 19 and 41 days in the presence and absence of the endogeic earthworm Aporrectodea caliginosa, respectively. [U- 14C]2,4-DCP mineralization was higher in cast, burrow wall and soil from columns with than without earthworms. [U- 14C]2,4-DCP mineralization was lowest in gut contents. Data indicated a strong impact of earthworms on the active microbial community. Novosphingobium, Comamonas and Desulfitobacterium sp. assimilated 2,4-DCP-[ 13C] in the absence of earthworms. Pseudomonas, Flavobacterium and Clostridium sp. assimilated 2,4-DCP-[ 13C] in the drilosphere. Novosphingobium- and Variovorax-related taxa dominated [U- 13C]2,4-DCP consumers in soil slurries with drilosphere and bulk soil material. 16S rRNA sequences suggested species level novelty. The collective data demonstrates that new Bacteroidetes and Alphaproteobacteria/Betaproteobacteria were involved in 2,4-DCP-C transformation and indicated that diverse and hitherto unknown microbes associated with carbon flow from 2,4-DCP are shaped by earthworms.

UR - http://www.scopus.com/inward/record.url?scp=84891623985&partnerID=8YFLogxK

U2 - 10.1111/1462-2920.12209

DO - 10.1111/1462-2920.12209

M3 - Article

VL - 16

SP - 84

EP - 100

JO - Environmental microbiology

JF - Environmental microbiology

SN - 1462-2912

IS - 1

ER -

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