Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 189-197 |
| Seitenumfang | 9 |
| Fachzeitschrift | BIOCONTROL |
| Jahrgang | 60 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - Apr. 2015 |
Abstract
Efficiency of natural enemies in pest control in the field frequently shows high degree of variability, making it difficult to propose reliable biocontrol strategies. Due to the small size of many beneficals, underlying mechanisms are difficult to evaluate. In the present study the potential of molecular markers to reconstruct individual foraging decisions of a small parasitoid species unsuitable for direct tracking, i.e. Diaeretiella rapae (M`Intosh) (Hymenoptera: Braconidae), a primary parasitoid of the cabbage aphid Brevicoryne brassicae (Linnaeus) (Hemiptera: Aphididae), was evaluated. Nine newly designed microsatellite primer pairs were tested for their reliability in parentage analysis. Therefore, several families of D.rapae with four to six daughters and high or low degree of inbreeding were established in the lab. Individuals were genotyped and parentage and sibship analysis was calculated with the software COLONY. The results indicated that 91 % of the 85 genotyped offspring from all families have been assigned to the correct family when all nine microsatellite loci have been used and 86 % when only six microsatellite loci have been used. Even in families with high degree of inbreeding up to 87 % of the offspring could be assigned correctly. In conclusion, the sibship analysis of D. rapae provides reliable results, especially with all nine selected microsatellite loci. Therefore it will be a valuable tool to reconstruct individual foraging decisions and characterize parasitoid “personality” in the field to improve biological control strategies.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Agrar- und Biowissenschaften (insg.)
- Insektenkunde
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in: BIOCONTROL, Jahrgang 60, Nr. 2, 04.2015, S. 189-197.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Adding “personality” to biocontrol
T2 - characterization and suitability of microsatellites for sibship reconstruction in the aphid parasitoid Diaeretiella rapae
AU - Eisele, Isabelle
AU - Meyhöfer, Rainer
N1 - Publisher Copyright: © 2014, International Organization for Biological Control (IOBC). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2015/4
Y1 - 2015/4
N2 - Efficiency of natural enemies in pest control in the field frequently shows high degree of variability, making it difficult to propose reliable biocontrol strategies. Due to the small size of many beneficals, underlying mechanisms are difficult to evaluate. In the present study the potential of molecular markers to reconstruct individual foraging decisions of a small parasitoid species unsuitable for direct tracking, i.e. Diaeretiella rapae (M`Intosh) (Hymenoptera: Braconidae), a primary parasitoid of the cabbage aphid Brevicoryne brassicae (Linnaeus) (Hemiptera: Aphididae), was evaluated. Nine newly designed microsatellite primer pairs were tested for their reliability in parentage analysis. Therefore, several families of D.rapae with four to six daughters and high or low degree of inbreeding were established in the lab. Individuals were genotyped and parentage and sibship analysis was calculated with the software COLONY. The results indicated that 91 % of the 85 genotyped offspring from all families have been assigned to the correct family when all nine microsatellite loci have been used and 86 % when only six microsatellite loci have been used. Even in families with high degree of inbreeding up to 87 % of the offspring could be assigned correctly. In conclusion, the sibship analysis of D. rapae provides reliable results, especially with all nine selected microsatellite loci. Therefore it will be a valuable tool to reconstruct individual foraging decisions and characterize parasitoid “personality” in the field to improve biological control strategies.
AB - Efficiency of natural enemies in pest control in the field frequently shows high degree of variability, making it difficult to propose reliable biocontrol strategies. Due to the small size of many beneficals, underlying mechanisms are difficult to evaluate. In the present study the potential of molecular markers to reconstruct individual foraging decisions of a small parasitoid species unsuitable for direct tracking, i.e. Diaeretiella rapae (M`Intosh) (Hymenoptera: Braconidae), a primary parasitoid of the cabbage aphid Brevicoryne brassicae (Linnaeus) (Hemiptera: Aphididae), was evaluated. Nine newly designed microsatellite primer pairs were tested for their reliability in parentage analysis. Therefore, several families of D.rapae with four to six daughters and high or low degree of inbreeding were established in the lab. Individuals were genotyped and parentage and sibship analysis was calculated with the software COLONY. The results indicated that 91 % of the 85 genotyped offspring from all families have been assigned to the correct family when all nine microsatellite loci have been used and 86 % when only six microsatellite loci have been used. Even in families with high degree of inbreeding up to 87 % of the offspring could be assigned correctly. In conclusion, the sibship analysis of D. rapae provides reliable results, especially with all nine selected microsatellite loci. Therefore it will be a valuable tool to reconstruct individual foraging decisions and characterize parasitoid “personality” in the field to improve biological control strategies.
KW - Braconidae
KW - COLONY software
KW - Foraging behavior
KW - Molecular marker
KW - Parentage
KW - Sibling analysis
UR - http://www.scopus.com/inward/record.url?scp=84925506842&partnerID=8YFLogxK
U2 - 10.1007/s10526-014-9643-2
DO - 10.1007/s10526-014-9643-2
M3 - Article
AN - SCOPUS:84925506842
VL - 60
SP - 189
EP - 197
JO - BIOCONTROL
JF - BIOCONTROL
SN - 1386-6141
IS - 2
ER -