TY - JOUR

T1 - Adaptation of in-situ microscopy for crystallization processes

AU - Bluma, A.

AU - Höpfner, T.

AU - Rudolph, G.

AU - Lindner, P.

AU - Beutel, S.

AU - Hitzmann, B.

AU - Scheper, T.

N1 - Funding information: The authors would like to thank the DFG for supporting the Project SCHE279/23-1.

PY - 2009/7/9

Y1 - 2009/7/9

N2 - In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.

AB - In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.

KW - A1. Biocrystallization

KW - A2. Crystal growth

KW - A2. Crystal morphology

KW - B1. ASS

KW - B1. Lysozyme

KW - B3. In-situ microscopy

UR - http://www.scopus.com/inward/record.url?scp=68549099695&partnerID=8YFLogxK

U2 - 10.1016/j.jcrysgro.2009.06.057

DO - 10.1016/j.jcrysgro.2009.06.057

M3 - Article

AN - SCOPUS:68549099695

VL - 311

SP - 4193

EP - 4198

JO - Journal of crystal growth

JF - Journal of crystal growth

SN - 0022-0248

IS - 17

ER -