TY - JOUR

T1 - A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-κB signaling in activated T cells

AU - Yin, Hongli

AU - Karayel, Ozge

AU - Chao, Ying Yin

AU - Seeholzer, Thomas

AU - Hamp, Isabel

AU - Plettenburg, Oliver

AU - Gehring, Torben

AU - Zielinski, Christina

AU - Mann, Matthias

AU - Krappmann, Daniel

N1 - Funding Information: Open Access funding enabled and organized by Projekt DEAL. Work of D.K. was supported by the Deutsche Forschungsgemeinschaft SFB1054 (A04, ID210592381) and SFB1335 (P07, ID360372040). Work of CZ was supported by the Deutsche Forschungsgemeinschaft SFB1054 (B10, ID210592381), SFB1335 (P18, ID360372040), Collaborative Research Centre (CRC)/Transregio124 FungiNet (C7, No 210879364) and the Carl-Zeiss-Stiftung.

PY - 2022/2

Y1 - 2022/2

N2 - T cell activation initiates protective adaptive immunity, but counterbalancing mechanisms are critical to prevent overshooting responses and to maintain immune homeostasis. The CARD11-BCL10-MALT1 (CBM) complex bridges T cell receptor engagement to NF-κB signaling and MALT1 protease activation. Here, we show that ABIN-1 is modulating the suppressive function of A20 in T cells. Using quantitative mass spectrometry, we identified ABIN-1 as an interactor of the CBM signalosome in activated T cells. A20 and ABIN-1 counteract inducible activation of human primary CD4 and Jurkat T cells. While A20 overexpression is able to silence CBM complex-triggered NF-κB and MALT1 protease activation independent of ABIN-1, the negative regulatory function of ABIN-1 depends on A20. The suppressive function of A20 in T cells relies on ubiquitin binding through the C-terminal zinc finger (ZnF)4/7 motifs, but does not involve the deubiquitinating activity of the OTU domain. Our mechanistic studies reveal that the A20/ABIN-1 module is recruited to the CBM complex via A20 ZnF4/7 and that proteasomal degradation of A20 and ABIN-1 releases the CBM complex from the negative impact of both regulators. Ubiquitin binding to A20 ZnF4/7 promotes destructive K48-polyubiquitination to itself and to ABIN-1. Further, after prolonged T cell stimulation, ABIN-1 antagonizes MALT1-catalyzed cleavage of re-synthesized A20 and thereby diminishes sustained CBM complex signaling. Taken together, interdependent post-translational mechanisms are tightly controlling expression and activity of the A20/ABIN-1 silencing module and the cooperative action of both negative regulators is critical to balance CBM complex signaling and T cell activation.

AB - T cell activation initiates protective adaptive immunity, but counterbalancing mechanisms are critical to prevent overshooting responses and to maintain immune homeostasis. The CARD11-BCL10-MALT1 (CBM) complex bridges T cell receptor engagement to NF-κB signaling and MALT1 protease activation. Here, we show that ABIN-1 is modulating the suppressive function of A20 in T cells. Using quantitative mass spectrometry, we identified ABIN-1 as an interactor of the CBM signalosome in activated T cells. A20 and ABIN-1 counteract inducible activation of human primary CD4 and Jurkat T cells. While A20 overexpression is able to silence CBM complex-triggered NF-κB and MALT1 protease activation independent of ABIN-1, the negative regulatory function of ABIN-1 depends on A20. The suppressive function of A20 in T cells relies on ubiquitin binding through the C-terminal zinc finger (ZnF)4/7 motifs, but does not involve the deubiquitinating activity of the OTU domain. Our mechanistic studies reveal that the A20/ABIN-1 module is recruited to the CBM complex via A20 ZnF4/7 and that proteasomal degradation of A20 and ABIN-1 releases the CBM complex from the negative impact of both regulators. Ubiquitin binding to A20 ZnF4/7 promotes destructive K48-polyubiquitination to itself and to ABIN-1. Further, after prolonged T cell stimulation, ABIN-1 antagonizes MALT1-catalyzed cleavage of re-synthesized A20 and thereby diminishes sustained CBM complex signaling. Taken together, interdependent post-translational mechanisms are tightly controlling expression and activity of the A20/ABIN-1 silencing module and the cooperative action of both negative regulators is critical to balance CBM complex signaling and T cell activation.

KW - CARMA1

KW - Immune activation

KW - Immune suppression

KW - T cell signaling

KW - TNFAIP3

KW - TNIP1

UR - http://www.scopus.com/inward/record.url?scp=85123904660&partnerID=8YFLogxK

U2 - 10.1007/s00018-022-04154-z

DO - 10.1007/s00018-022-04154-z

M3 - Article

C2 - 35099607

AN - SCOPUS:85123904660

VL - 79

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-682X

IS - 2

M1 - 112

ER -