Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 3447 |
| Fachzeitschrift | Nature Communications |
| Jahrgang | 5 |
| Publikationsstatus | Veröffentlicht - 11 März 2014 |
| Extern publiziert | Ja |
Abstract
Transcription activator-like effectors (TALEs) are important Xanthomonas virulence factors that bind DNA via a unique tandem 34-amino-acid repeat domain to induce expression of plant genes. So far, TALE repeats are described to bind as a consecutive array to a consecutive DNA sequence, in which each repeat independently recognizes a single DNA base. This modular protein architecture enables the design of any desired DNA-binding specificity for biotechnology applications. Here we report that natural TALE repeats of unusual amino-acid sequence length break the strict one repeat-to-one base pair binding mode and introduce a local flexibility to TALE-DNA binding. This flexibility allows TALEs and TALE nucleases to recognize target sequence variants with single nucleotide deletions. The flexibility also allows TALEs to activate transcription at allelic promoters that otherwise confer resistance to the host plant.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Allgemeine Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Allgemeine Biochemie, Genetik und Molekularbiologie
- Physik und Astronomie (insg.)
- Allgemeine Physik und Astronomie
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in: Nature Communications, Jahrgang 5, 3447, 11.03.2014.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A TAL effector repeat architecture for frameshift binding
AU - Richter, Annekatrin
AU - Streubel, Jana
AU - Blücher, Christina
AU - Szurek, Boris
AU - Reschke, Maik
AU - Grau, Jan
AU - Boch, Jens
N1 - Funding Information: We thank T. Schreiber, S. Thieme and U. Bonas for providing the GoldenGate-compatible vectors; K. Schlien for extending the Golden TAL kit, ArtXa70 and ArtXa71 constructs; T. Schreiber and U. Bonas for suggestions on the manuscript; R. Szczesny for providing FokI constructs; J. Leach for providing avrXa7; C. Kretschmer for technical assistance and U. Bonas for support. This work was supported by grants from the Deutsche Forschungsgemeinschaft (SPP 1212, BO 1496/6-1, BO 1496/7-1), and from the European Regional Development Fund of the European Commission. B.S. is supported by a grant from GRiSP for New Frontiers Research projects (MENERGEP). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/3/11
Y1 - 2014/3/11
N2 - Transcription activator-like effectors (TALEs) are important Xanthomonas virulence factors that bind DNA via a unique tandem 34-amino-acid repeat domain to induce expression of plant genes. So far, TALE repeats are described to bind as a consecutive array to a consecutive DNA sequence, in which each repeat independently recognizes a single DNA base. This modular protein architecture enables the design of any desired DNA-binding specificity for biotechnology applications. Here we report that natural TALE repeats of unusual amino-acid sequence length break the strict one repeat-to-one base pair binding mode and introduce a local flexibility to TALE-DNA binding. This flexibility allows TALEs and TALE nucleases to recognize target sequence variants with single nucleotide deletions. The flexibility also allows TALEs to activate transcription at allelic promoters that otherwise confer resistance to the host plant.
AB - Transcription activator-like effectors (TALEs) are important Xanthomonas virulence factors that bind DNA via a unique tandem 34-amino-acid repeat domain to induce expression of plant genes. So far, TALE repeats are described to bind as a consecutive array to a consecutive DNA sequence, in which each repeat independently recognizes a single DNA base. This modular protein architecture enables the design of any desired DNA-binding specificity for biotechnology applications. Here we report that natural TALE repeats of unusual amino-acid sequence length break the strict one repeat-to-one base pair binding mode and introduce a local flexibility to TALE-DNA binding. This flexibility allows TALEs and TALE nucleases to recognize target sequence variants with single nucleotide deletions. The flexibility also allows TALEs to activate transcription at allelic promoters that otherwise confer resistance to the host plant.
UR - http://www.scopus.com/inward/record.url?scp=84896380709&partnerID=8YFLogxK
U2 - 10.1038/ncomms4447
DO - 10.1038/ncomms4447
M3 - Article
C2 - 24614980
AN - SCOPUS:84896380709
VL - 5
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 3447
ER -