TY - JOUR

T1 - A Simple Method for the Isolation and Purification of L-Asparaginase from Enterobacter aerogenes

AU - Mukherjee, J.

AU - Joeris, K.

AU - Riechel, P.

AU - Scheper, T.

N1 - Funding information: The first author wishes to thank the Deutscher Akademischer Austauschdienst and the Gottlieb Daimler und Karl Benz Stifiung for their financial support.

PY - 1999/2

Y1 - 1999/2

N2 - L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54 %. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.

AB - L-Asparaginase from Enterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5-6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54 %. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification of L-asparaginase. The choice of the column material was critical in the purification process.

UR - http://www.scopus.com/inward/record.url?scp=0032616756&partnerID=8YFLogxK

U2 - 10.1007/BF02816214

DO - 10.1007/BF02816214

M3 - Article

C2 - 10489691

AN - SCOPUS:0032616756

VL - 44

SP - 15

EP - 18

JO - Folia microbiologica

JF - Folia microbiologica

SN - 0015-5632

IS - 1

ER -