Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 863-869 |
| Seitenumfang | 7 |
| Fachzeitschrift | Analytical and Bioanalytical Chemistry |
| Jahrgang | 394 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 24 März 2009 |
Abstract
An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 μg•l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
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in: Analytical and Bioanalytical Chemistry, Jahrgang 394, Nr. 3, 24.03.2009, S. 863-869.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A semi-quantitative dipstick assay for microcystin
AU - Tippkötter, Nils
AU - Stückmann, Henning
AU - Kroll, Stephen
AU - Winkelmann, Gunda
AU - Noack, Udo
AU - Scheper, Thomas
AU - Ulber, Roland
PY - 2009/3/24
Y1 - 2009/3/24
N2 - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 μg•l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.
AB - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 μg•l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.
KW - Dipstick
KW - Gold nanoparticles
KW - Immunochromatographic
KW - Lateral flow
KW - Microcystin
KW - Toxin
UR - http://www.scopus.com/inward/record.url?scp=67349156116&partnerID=8YFLogxK
U2 - 10.1007/s00216-009-2750-8
DO - 10.1007/s00216-009-2750-8
M3 - Article
C2 - 19306114
AN - SCOPUS:67349156116
VL - 394
SP - 863
EP - 869
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 3
ER -