TY - JOUR

T1 - A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa

AU - Roche, Béatrice

AU - Garcia-Rivera, Mariel A.

AU - Normant, Vincent

AU - Kuhn, Lauriane

AU - Hammann, Philippe

AU - Brönstrup, Mark

AU - Mislin, Gaëtan L.A.

AU - Schalk, Isabelle J.

N1 - Funding Information: Authors acknowledge the Centre National de la Recherche Scientifique (CNRS) for general financial support. BR would like to thank Roche Pharmaceutical Research and Early Development Basel for their financial support via the Roche Postdoctoral Fellowship (RPF) Program. We thank Aurélia Battesti (LCB, Marseille) for kindly providing the empty two‐hybrid vectors. The mass spectrometry instrumentation was funded by the University of Strasbourg, IdEx 'Equipement mi‐lourd' 2015. We also received support by a grant from the Joint Programming Initiative on Antimicrobial Resistance (JPI AMR, grant number: 01K11825).

PY - 2022/2/25

Y1 - 2022/2/25

N2 - Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55Fe uptake assays, and LC–MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.

AB - Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55Fe uptake assays, and LC–MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.

UR - http://www.scopus.com/inward/record.url?scp=85117180587&partnerID=8YFLogxK

U2 - 10.1111/1462-2920.15811

DO - 10.1111/1462-2920.15811

M3 - Article

AN - SCOPUS:85117180587

VL - 24

SP - 866

EP - 877

JO - Environmental microbiology

JF - Environmental microbiology

SN - 1462-2912

IS - 2

ER -