A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

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  • NIFE- Niedersächsisches Zentrum für Biomedizintechnik, Implantatforschung und Entwicklung
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Details

OriginalspracheEnglisch
Aufsatznummer109095
FachzeitschriftBiochemical engineering journal
Jahrgang200
Frühes Online-Datum27 Sept. 2023
PublikationsstatusVeröffentlicht - Nov. 2023

Abstract

Pre-endothelialization of a tissue-engineered vascular graft before implantation aims to prevent thrombosis and immunoreactions. This work demonstrates a standardized cultivation process to build a confluent monolayer with human aortal endothelial cells on xenogenous scaffolds. Pre-tested dynamic cultivation conditions in flow slides with pulsatile flow (1 Hz) representing arterial wall conditions were transferred to a newly designed multi-featured rotational bioreactor system. The medium was thickened with 1% methyl cellulose simulating a non-Newtonian fluid comparable to blood. Computational fluid dynamics was used to estimate the optimal volume flow and medium distribution inside the bioreactor chamber for defined wall-near shear stress levels. Flow measurements were performed during cultivation for constant monitoring of the process. Three decellularized porcine arteries were seeded and cultivated in the bioreactor over six days. 1% MC turned out to be the optimal percentage to achieve shear stress values ranging up to 10 dyn/cm 2. Vascular endothelial cells formed a continuous monolayer with significant cell alignment in the direction of flow. The presented cultivation protocol in the bioreactor system thus displays a promising template for graft endothelialization and cultivation. Therefore, establishing a key step for future tissue-engineered vascular graft development with a view towards clinical application.

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A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application. / Heene, Sebastian; Renzelmann, Jannis; Müller, Caroline et al.
in: Biochemical engineering journal, Jahrgang 200, 109095, 11.2023.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Heene S, Renzelmann J, Müller C, Stanislawski N, Cholewa F, Moosmann P et al. A promising protocol for the endothelialization of vascular grafts in an instrumented rotating bioreactor towards clinical application. Biochemical engineering journal. 2023 Nov;200:109095. Epub 2023 Sep 27. doi: 10.1016/j.bej.2023.109095
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AU - Heene, Sebastian

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AU - Müller, Caroline

AU - Stanislawski, Nils

AU - Cholewa, Fabian

AU - Moosmann, Pia

AU - Blume, Holger

AU - Blume, Cornelia

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