Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 581-586 |
| Seitenumfang | 6 |
| Fachzeitschrift | Biotechnology progress |
| Jahrgang | 28 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 18 Okt. 2011 |
Abstract
The newly developed in situ oxygen uptake rate (in situ OUR) probe presented in this article is based on the in situ microscope technology platform. It is designed to measure the oxygen uptake rate (OUR) of mammalian cells, an important parameter for metabolic flux analysis, inside a reactor (in situ) and in real-time. The system isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time, and releases the sample again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard dissolved oxygen (DO) probe for process monitoring when it is not performing OUR measurements. It can be equipped with two different types of optical sensors (i.e., DO, pH) simultaneously or a conventional polarographic DO-probe (Clark type). This new probe was successfully tested in baby hamster kidney perfusion cell cultures.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
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in: Biotechnology progress, Jahrgang 28, Nr. 2, 18.10.2011, S. 581-586.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A Novel In Situ Probe for Oxygen Uptake Rate Measurement inMammalian Cell Cultures
AU - Behr, Larissa
AU - Joeris, Klaus
AU - Burnett, Mark
AU - Scheper, Thomas
PY - 2011/10/18
Y1 - 2011/10/18
N2 - The newly developed in situ oxygen uptake rate (in situ OUR) probe presented in this article is based on the in situ microscope technology platform. It is designed to measure the oxygen uptake rate (OUR) of mammalian cells, an important parameter for metabolic flux analysis, inside a reactor (in situ) and in real-time. The system isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time, and releases the sample again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard dissolved oxygen (DO) probe for process monitoring when it is not performing OUR measurements. It can be equipped with two different types of optical sensors (i.e., DO, pH) simultaneously or a conventional polarographic DO-probe (Clark type). This new probe was successfully tested in baby hamster kidney perfusion cell cultures.
AB - The newly developed in situ oxygen uptake rate (in situ OUR) probe presented in this article is based on the in situ microscope technology platform. It is designed to measure the oxygen uptake rate (OUR) of mammalian cells, an important parameter for metabolic flux analysis, inside a reactor (in situ) and in real-time. The system isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time, and releases the sample again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard dissolved oxygen (DO) probe for process monitoring when it is not performing OUR measurements. It can be equipped with two different types of optical sensors (i.e., DO, pH) simultaneously or a conventional polarographic DO-probe (Clark type). This new probe was successfully tested in baby hamster kidney perfusion cell cultures.
KW - Bioprocess monitoring
KW - In situ microscopy
KW - In situ oxygen uptake rate
KW - Mammalian cell cultivation
KW - Sensor systems
UR - http://www.scopus.com/inward/record.url?scp=84859641380&partnerID=8YFLogxK
U2 - 10.1002/btpr.741
DO - 10.1002/btpr.741
M3 - Article
C2 - 22083967
AN - SCOPUS:84859641380
VL - 28
SP - 581
EP - 586
JO - Biotechnology progress
JF - Biotechnology progress
SN - 8756-7938
IS - 2
ER -