TY - JOUR

T1 - A new set up for multi-analyte sensing: At-line bio-process monitoring

AU - Akin, Mehriban

AU - Prediger, Andreas

AU - Yuksel, Merve

AU - Höpfner, Tim

AU - Demirkol, Dilek Odaci

AU - Beutel, Sascha

AU - Timur, Suna

AU - Scheper, Thomas

N1 - Funding information: This work is supported by Scientific and Technological Research Council of Turkey (TUBITAK) under the Research Project number 108T293 and Federal Ministry of Education and Research (BMBF) under the project number TUR08/004. Ege University, Research and Application Center of Science and Technology (EBILTEM) , is acknowledged for the research grants (Project number; 2009 BIL 008).

PY - 2011/5/19

Y1 - 2011/5/19

N2 - A multi-analyte sensing device is described, for simultaneous at-line monitoring of glucose, ethanol, pO 2-value and cell density. It consists of a dual biosensor, a modified microscope and a fiber optical pO 2-sensor that are integrated into a flow analysis (FA) system.The biosensor is based on a conventional thin layer flow-through cell equipped with a gold (Au) dual electrode (serial configuration). The biosensors with no cross-talking were produced by modifying the electrochemical transducers. Each Au surface was initially modified by self-assembled monolayer (SAM) of cysteamine. Alcohol oxidase (AOx) and pyranose oxidase (PyOx) were immobilized each onto a gold surface by means of PAMAM (polyamidoamine) dendrimer via glutaraldehyde cross-linking. The responses for glucose and ethanol were linear up to 0.5. mM. The operational stability of the biosensors was very promising, after 11. h continuous operation, only 6.0% of the initial activity was lost. The potential of the described biosensor was demonstrated by parallel determination of ethanol and glucose in yeast fermentation process.Simultaneously the cell density of the culture was monitored with an in situ microscope (ISM), which was integrated into the FA system. Both the used in situ microscope and the image processing algorithm used for the analysis of the acquired image data are described. Furthermore the pO 2-value was monitored using a fiber optical sensor, which was embedded in a flow cell. The multi-sensor device allows the at-line monitoring of several process values without the need for further sampling or time consuming offline measurements.

AB - A multi-analyte sensing device is described, for simultaneous at-line monitoring of glucose, ethanol, pO 2-value and cell density. It consists of a dual biosensor, a modified microscope and a fiber optical pO 2-sensor that are integrated into a flow analysis (FA) system.The biosensor is based on a conventional thin layer flow-through cell equipped with a gold (Au) dual electrode (serial configuration). The biosensors with no cross-talking were produced by modifying the electrochemical transducers. Each Au surface was initially modified by self-assembled monolayer (SAM) of cysteamine. Alcohol oxidase (AOx) and pyranose oxidase (PyOx) were immobilized each onto a gold surface by means of PAMAM (polyamidoamine) dendrimer via glutaraldehyde cross-linking. The responses for glucose and ethanol were linear up to 0.5. mM. The operational stability of the biosensors was very promising, after 11. h continuous operation, only 6.0% of the initial activity was lost. The potential of the described biosensor was demonstrated by parallel determination of ethanol and glucose in yeast fermentation process.Simultaneously the cell density of the culture was monitored with an in situ microscope (ISM), which was integrated into the FA system. Both the used in situ microscope and the image processing algorithm used for the analysis of the acquired image data are described. Furthermore the pO 2-value was monitored using a fiber optical sensor, which was embedded in a flow cell. The multi-sensor device allows the at-line monitoring of several process values without the need for further sampling or time consuming offline measurements.

KW - Biosensor

KW - In situ microscopy

KW - Multi-analyte sensing

KW - Yeast fermentation

UR - http://www.scopus.com/inward/record.url?scp=79959230564&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2011.05.018

DO - 10.1016/j.bios.2011.05.018

M3 - Article

C2 - 21664121

AN - SCOPUS:79959230564

VL - 26

SP - 4532

EP - 4537

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

IS - 11

ER -