TY - JOUR

T1 - A morphology-based model for fed-batch cultivations of Penicillium chrysogenum growing in pellet form

AU - Meyerhoff, Juliane

AU - Bellgardt, Karl heinz

PY - 1995/1/31

Y1 - 1995/1/31

N2 - A model that was employed in simulations of the development of single fungal pellets was extended in order to describe Penicillium chrysogenum fed-batch cultivations. Spore germination, pre- and main culture of up to 100 pellets were simulated, each of which represents a subpopulation of the entire biomass. Experimentally observed pellet size distributions at the beginning of the main cultivation are explained by different times of spore germination. In the model, penicillin production was coupled to the morphology (active cells not containing tips) and described by a simple first-order rate equation. The experimental data of biomass, glucose, penicillin concentration, and pellet size distribution could only be described if pellet fragmentation was included in the model, which also explains the decreasing pellet size during cultivation in a stirred tank. It was assumed that the growth activity of pellet fragments is impaired because of cell damage, repair activities and other influences. The model was submitted to a sensitivity analysis and compared to a previously presented segregated model.

AB - A model that was employed in simulations of the development of single fungal pellets was extended in order to describe Penicillium chrysogenum fed-batch cultivations. Spore germination, pre- and main culture of up to 100 pellets were simulated, each of which represents a subpopulation of the entire biomass. Experimentally observed pellet size distributions at the beginning of the main cultivation are explained by different times of spore germination. In the model, penicillin production was coupled to the morphology (active cells not containing tips) and described by a simple first-order rate equation. The experimental data of biomass, glucose, penicillin concentration, and pellet size distribution could only be described if pellet fragmentation was included in the model, which also explains the decreasing pellet size during cultivation in a stirred tank. It was assumed that the growth activity of pellet fragments is impaired because of cell damage, repair activities and other influences. The model was submitted to a sensitivity analysis and compared to a previously presented segregated model.

KW - Fed-batch cultivation

KW - Model

KW - Morphology

KW - Pellet

KW - Shear force

UR - http://www.scopus.com/inward/record.url?scp=0028888256&partnerID=8YFLogxK

U2 - 10.1016/0168-1656(94)00121-R

DO - 10.1016/0168-1656(94)00121-R

M3 - Article

AN - SCOPUS:0028888256

VL - 38

SP - 201

EP - 217

JO - Journal of biotechnology

JF - Journal of biotechnology

SN - 0168-1656

IS - 3

ER -