Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 387-393 |
| Seitenumfang | 7 |
| Fachzeitschrift | Biotechnology and Bioengineering |
| Jahrgang | 42 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - Juli 1993 |
| Extern publiziert | Ja |
Abstract
Using permeabilized Zymomonas mobilis or glucose–fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzyme were confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a range from 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocess monitoring was proved using samples from a Pseudomonas pseudoflava cultivation. © 1993 John Wiley & Sons, Inc.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Biotechnology and Bioengineering, Jahrgang 42, Nr. 3, 07.1993, S. 387-393.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A Model System for a Fluorometric Biosensor Using Permeabilized Zymomonas mobilis or Enzymes with Protein Confined Dinucleotides
AU - Thordsen, O.
AU - Lee, S. J.
AU - Degelau, A.
AU - Scheper, T.
AU - Loos, H.
AU - Rehr, B.
AU - Sahm, H.
PY - 1993/7
Y1 - 1993/7
N2 - Using permeabilized Zymomonas mobilis or glucose–fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzyme were confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a range from 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocess monitoring was proved using samples from a Pseudomonas pseudoflava cultivation. © 1993 John Wiley & Sons, Inc.
AB - Using permeabilized Zymomonas mobilis or glucose–fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzyme were confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a range from 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocess monitoring was proved using samples from a Pseudomonas pseudoflava cultivation. © 1993 John Wiley & Sons, Inc.
KW - bioprocess monitoring
KW - biosensor
KW - fluorescence
KW - fructose
KW - gluconolactone
KW - glucose
KW - NADPH
KW - permeabilization
KW - sorbitol
KW - Zymomonas mobilis
UR - http://www.scopus.com/inward/record.url?scp=0027624273&partnerID=8YFLogxK
U2 - 10.1002/bit.260420317
DO - 10.1002/bit.260420317
M3 - Article
AN - SCOPUS:0027624273
VL - 42
SP - 387
EP - 393
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 3
ER -