Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 533-541 |
| Seitenumfang | 9 |
| Fachzeitschrift | Applied Microbiology and Biotechnology |
| Jahrgang | 78 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 1 März 2008 |
Abstract
The conversion of β-myrcene to the furanoid flavour compound perillene by Pleurotus ostreatus was investigated using trideutero β-myrcene, trideutero α-(Z)-acaridiol and non-labeled 1,2- and 3,10-epoxy-β- myrcene, α,α-acarilactol, and perillene as substrates. Myrcene diols were formed from the cleavage of myrcene epoxides, but only α-(Z)-acaridiol, a 1,4-butanediol derivative most likely generated through a base-catalysed epoxide opening, was a suitable precursor of perillene. Once formed, this key intermediate was rapidly oxidised and the resulting cyclic lactol was dehydrated to yield perillene. Bioconversion of the supplemented perillene to α,α-acariolide indicated that perillene was another intermediate of the pathway and prone to further oxidative degradation. The data suggest that the fungus converted the cytotoxic β-myrcene in its environment into a metabolically useable carbon source along this route.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Applied Microbiology and Biotechnology, Jahrgang 78, Nr. 3, 01.03.2008, S. 533-541.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A labeling study on the formation of perillene by submerged cultured oyster mushroom, Pleurotus ostreatus
AU - Krings, Ulrich
AU - Hapetta, Darius
AU - Berger, Ralf G.
N1 - Funding information: Acknowledgement We are grateful for a grant from the Deutsche Forschungsgemeinschaft (DFG KR 2958/1-1).
PY - 2008/3/1
Y1 - 2008/3/1
N2 - The conversion of β-myrcene to the furanoid flavour compound perillene by Pleurotus ostreatus was investigated using trideutero β-myrcene, trideutero α-(Z)-acaridiol and non-labeled 1,2- and 3,10-epoxy-β- myrcene, α,α-acarilactol, and perillene as substrates. Myrcene diols were formed from the cleavage of myrcene epoxides, but only α-(Z)-acaridiol, a 1,4-butanediol derivative most likely generated through a base-catalysed epoxide opening, was a suitable precursor of perillene. Once formed, this key intermediate was rapidly oxidised and the resulting cyclic lactol was dehydrated to yield perillene. Bioconversion of the supplemented perillene to α,α-acariolide indicated that perillene was another intermediate of the pathway and prone to further oxidative degradation. The data suggest that the fungus converted the cytotoxic β-myrcene in its environment into a metabolically useable carbon source along this route.
AB - The conversion of β-myrcene to the furanoid flavour compound perillene by Pleurotus ostreatus was investigated using trideutero β-myrcene, trideutero α-(Z)-acaridiol and non-labeled 1,2- and 3,10-epoxy-β- myrcene, α,α-acarilactol, and perillene as substrates. Myrcene diols were formed from the cleavage of myrcene epoxides, but only α-(Z)-acaridiol, a 1,4-butanediol derivative most likely generated through a base-catalysed epoxide opening, was a suitable precursor of perillene. Once formed, this key intermediate was rapidly oxidised and the resulting cyclic lactol was dehydrated to yield perillene. Bioconversion of the supplemented perillene to α,α-acariolide indicated that perillene was another intermediate of the pathway and prone to further oxidative degradation. The data suggest that the fungus converted the cytotoxic β-myrcene in its environment into a metabolically useable carbon source along this route.
KW - β-myrcene
KW - Bioconversion
KW - Labeling study
KW - Perillene
KW - Pleurotus ostreatus
UR - http://www.scopus.com/inward/record.url?scp=39149101459&partnerID=8YFLogxK
U2 - 10.1007/s00253-007-1335-8
DO - 10.1007/s00253-007-1335-8
M3 - Article
C2 - 18196236
AN - SCOPUS:39149101459
VL - 78
SP - 533
EP - 541
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 3
ER -