A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autorschaft

  • Christof Dietrich
  • Qusai Al Abdallah
  • Lara Lintl
  • Agnes Pietruszka
  • Edgar Maiss

Organisationseinheiten

Externe Organisationen

  • Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V.Hans-Knöll-Institut
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)2846-2851
Seitenumfang6
FachzeitschriftJournal of General Virology
Jahrgang88
Ausgabenummer10
PublikationsstatusVeröffentlicht - 1 Okt. 2007

Abstract

The effect of a recombination event in the genomic 3′ end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3′ non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.

ASJC Scopus Sachgebiete

  • Immunologie und Mikrobiologie (insg.)
  • Virologie

Zitieren

A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. / Dietrich, Christof; Abdallah, Qusai Al; Lintl, Lara et al.
in: Journal of General Virology, Jahrgang 88, Nr. 10, 01.10.2007, S. 2846-2851.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Dietrich C, Abdallah QA, Lintl L, Pietruszka A, Maiss E. A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. Journal of General Virology. 2007 Okt 1;88(10):2846-2851. doi: 10.1099/vir.0.82989-0
Dietrich, Christof ; Abdallah, Qusai Al ; Lintl, Lara et al. / A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. in: Journal of General Virology. 2007 ; Jahrgang 88, Nr. 10. S. 2846-2851.
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