Details
Originalsprache | Englisch |
---|---|
Seiten (von - bis) | 703-707 |
Seitenumfang | 5 |
Fachzeitschrift | Journal of Chemical Engineering of Japan |
Jahrgang | 45 |
Ausgabenummer | 9 |
Publikationsstatus | Veröffentlicht - 20 Sept. 2012 |
Extern publiziert | Ja |
Abstract
Dynamic pulse experiments for cell analysis require rapid and precise preparation of probes, which is often not possible in a macro-laboratory environment. Lab-on-a-Chip technology can offer new ways of probe preparation for both chemical and biochemical processes. A passive microfluidic mixer (micromixer) is presented in this contribution, which is designed for the preparation of cells. The micromixer is based on the method of split-and-recombination. Two alternating channel layers result in a three-dimensional pathway. Mixing in the laminar flow regime not only relies on molecular diffusion but is also enhanced by chaotic advection. The mixer was fabricated in a glass-silicon-glass sandwich technology, and mixing was characterized by chemical and biological probes. The contribution of chaotic advection, which appears in repeated 90° turns of the channel geometry, could be confirmed in computational fluid dynamics (CFD) analysis and laser-induced fluorescence images. Mixing performance was characterized by chemical iodometry. This method is based on the chemical reaction of Lugol's solution and sodium thiosulfate. The resulting solution changes its color such that mixing becomes visible in a fluidic channel. Experiments were conducted for flow rates between 20μL/min and 1000μL/min corresponding to Reynolds numbers from 0.9 to 62. The experiments showed that fewer mixer units are necessary at higher flow rates because vorticity increases at higher Re in the recombination regions of the mixer. A mixing time of approximately 5 ms was achieved at a flow rate of 1000 μL/min at both inlets, which corresponds to a Re of 62 in this channel geometry. Biological pulse experiments were performed with the mixer, showing its suitability for preparing biological particles as eukaryotic cells.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Allgemeine Chemie
- Chemische Verfahrenstechnik (insg.)
- Allgemeine chemische Verfahrenstechnik
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in: Journal of Chemical Engineering of Japan, Jahrgang 45, Nr. 9, 20.09.2012, S. 703-707.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A chaotic advection enhanced microfluidic split-and-recombine mixer for the preparation of chemical and biological probes
AU - Rajabi, Negar
AU - Hoffmann, Marko
AU - Bahnemann, Janina
AU - Zeng, An Ping
AU - Schlüter, Michael
AU - Müller, Jörg
PY - 2012/9/20
Y1 - 2012/9/20
N2 - Dynamic pulse experiments for cell analysis require rapid and precise preparation of probes, which is often not possible in a macro-laboratory environment. Lab-on-a-Chip technology can offer new ways of probe preparation for both chemical and biochemical processes. A passive microfluidic mixer (micromixer) is presented in this contribution, which is designed for the preparation of cells. The micromixer is based on the method of split-and-recombination. Two alternating channel layers result in a three-dimensional pathway. Mixing in the laminar flow regime not only relies on molecular diffusion but is also enhanced by chaotic advection. The mixer was fabricated in a glass-silicon-glass sandwich technology, and mixing was characterized by chemical and biological probes. The contribution of chaotic advection, which appears in repeated 90° turns of the channel geometry, could be confirmed in computational fluid dynamics (CFD) analysis and laser-induced fluorescence images. Mixing performance was characterized by chemical iodometry. This method is based on the chemical reaction of Lugol's solution and sodium thiosulfate. The resulting solution changes its color such that mixing becomes visible in a fluidic channel. Experiments were conducted for flow rates between 20μL/min and 1000μL/min corresponding to Reynolds numbers from 0.9 to 62. The experiments showed that fewer mixer units are necessary at higher flow rates because vorticity increases at higher Re in the recombination regions of the mixer. A mixing time of approximately 5 ms was achieved at a flow rate of 1000 μL/min at both inlets, which corresponds to a Re of 62 in this channel geometry. Biological pulse experiments were performed with the mixer, showing its suitability for preparing biological particles as eukaryotic cells.
AB - Dynamic pulse experiments for cell analysis require rapid and precise preparation of probes, which is often not possible in a macro-laboratory environment. Lab-on-a-Chip technology can offer new ways of probe preparation for both chemical and biochemical processes. A passive microfluidic mixer (micromixer) is presented in this contribution, which is designed for the preparation of cells. The micromixer is based on the method of split-and-recombination. Two alternating channel layers result in a three-dimensional pathway. Mixing in the laminar flow regime not only relies on molecular diffusion but is also enhanced by chaotic advection. The mixer was fabricated in a glass-silicon-glass sandwich technology, and mixing was characterized by chemical and biological probes. The contribution of chaotic advection, which appears in repeated 90° turns of the channel geometry, could be confirmed in computational fluid dynamics (CFD) analysis and laser-induced fluorescence images. Mixing performance was characterized by chemical iodometry. This method is based on the chemical reaction of Lugol's solution and sodium thiosulfate. The resulting solution changes its color such that mixing becomes visible in a fluidic channel. Experiments were conducted for flow rates between 20μL/min and 1000μL/min corresponding to Reynolds numbers from 0.9 to 62. The experiments showed that fewer mixer units are necessary at higher flow rates because vorticity increases at higher Re in the recombination regions of the mixer. A mixing time of approximately 5 ms was achieved at a flow rate of 1000 μL/min at both inlets, which corresponds to a Re of 62 in this channel geometry. Biological pulse experiments were performed with the mixer, showing its suitability for preparing biological particles as eukaryotic cells.
KW - Biological Pulse Experiments
KW - Chaotic Advection
KW - Lab-on-a-Chip
KW - Microfluidics
KW - Micromixer
UR - http://www.scopus.com/inward/record.url?scp=84867866703&partnerID=8YFLogxK
U2 - 10.1252/jcej.12we071
DO - 10.1252/jcej.12we071
M3 - Article
AN - SCOPUS:84867866703
VL - 45
SP - 703
EP - 707
JO - Journal of Chemical Engineering of Japan
JF - Journal of Chemical Engineering of Japan
SN - 0021-9592
IS - 9
ER -