Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 801-812 |
| Seitenumfang | 12 |
| Fachzeitschrift | Turkish journal of chemistry |
| Jahrgang | 39 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 29 Apr. 2015 |
Abstract
The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025-1.0 mg/mL and 1.0-100 μg/mL concentrations for 24 h, the IC50values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.
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- Allgemeine Chemie
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in: Turkish journal of chemistry, Jahrgang 39, Nr. 4, 29.04.2015, S. 801-812.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil
AU - Ağ Şeleci, Didem
AU - Gümüş, Zinar Pinar
AU - Yavuz, Murat
AU - Şeleci, Muharrem
AU - Bongartz, Rebecca
AU - Stahl, Frank
AU - Coşkunol, Hakan
AU - Timur, Suna
AU - Scheper, Thomas
PY - 2015/4/29
Y1 - 2015/4/29
N2 - The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025-1.0 mg/mL and 1.0-100 μg/mL concentrations for 24 h, the IC50values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.
AB - The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025-1.0 mg/mL and 1.0-100 μg/mL concentrations for 24 h, the IC50values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.
KW - Black cumin seed oil
KW - Cell culture investigations
KW - Wheat germ oil
UR - http://www.scopus.com/inward/record.url?scp=84938305229&partnerID=8YFLogxK
U2 - 10.3906/kim-1502-72
DO - 10.3906/kim-1502-72
M3 - Article
AN - SCOPUS:84938305229
VL - 39
SP - 801
EP - 812
JO - Turkish journal of chemistry
JF - Turkish journal of chemistry
SN - 1300-0527
IS - 4
ER -