A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Christopher Heuer
  • John Alexander Preuss
  • Marc Buttkewitz
  • Thomas Scheper
  • Ester Segal
  • Janina Bahnemann

Organisationseinheiten

Externe Organisationen

  • Technion-Israel Institute of Technology
  • Universität Augsburg
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)4950-4961
Seitenumfang12
FachzeitschriftLAB on a chip
Jahrgang22
Ausgabenummer24
PublikationsstatusVeröffentlicht - 22 Nov. 2022

Abstract

With antimicrobial resistance becoming a major threat to healthcare settings around the world, there is a paramount need for rapid point-of-care antimicrobial susceptibility testing (AST) diagnostics. Unfortunately, most currently available clinical AST tools are lengthy, laborious, or are simply inappropriate for point-of-care testing. Herein, we design a 3D-printed microfluidic gradient generator that automatically produces two-fold dilution series of clinically relevant antimicrobials. We first establish the compatibility of these generators for classical AST (i.e., broth microdilution) and then extend their application to include a complete on-chip label-free and phenotypic AST. This is accomplished by the integration of photonic silicon chips, which provide a preferential surface for microbial colonization and allow optical tracking of bacterial behavior and growth at a solid-liquid interface in real-time by phase shift reflectometric interference spectroscopic measurements (PRISM). Using Escherichia coli and ciprofloxacin as a model pathogen-drug combination, we successfully determine the minimum inhibitory concentration within less than 90 minutes. This gradient generator-based PRISM assay provides an integrated AST device that is viable for convenient point-of-care testing and offers a promising and most importantly, rapid alternative to current clinical practices, which extend to 8-24 h.

ASJC Scopus Sachgebiete

Zitieren

A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing. / Heuer, Christopher; Preuss, John Alexander; Buttkewitz, Marc et al.
in: LAB on a chip, Jahrgang 22, Nr. 24, 22.11.2022, S. 4950-4961.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Heuer C, Preuss JA, Buttkewitz M, Scheper T, Segal E, Bahnemann J. A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing. LAB on a chip. 2022 Nov 22;22(24):4950-4961. doi: 10.1039/d2lc00640e
Heuer, Christopher ; Preuss, John Alexander ; Buttkewitz, Marc et al. / A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing. in: LAB on a chip. 2022 ; Jahrgang 22, Nr. 24. S. 4950-4961.
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abstract = "With antimicrobial resistance becoming a major threat to healthcare settings around the world, there is a paramount need for rapid point-of-care antimicrobial susceptibility testing (AST) diagnostics. Unfortunately, most currently available clinical AST tools are lengthy, laborious, or are simply inappropriate for point-of-care testing. Herein, we design a 3D-printed microfluidic gradient generator that automatically produces two-fold dilution series of clinically relevant antimicrobials. We first establish the compatibility of these generators for classical AST (i.e., broth microdilution) and then extend their application to include a complete on-chip label-free and phenotypic AST. This is accomplished by the integration of photonic silicon chips, which provide a preferential surface for microbial colonization and allow optical tracking of bacterial behavior and growth at a solid-liquid interface in real-time by phase shift reflectometric interference spectroscopic measurements (PRISM). Using Escherichia coli and ciprofloxacin as a model pathogen-drug combination, we successfully determine the minimum inhibitory concentration within less than 90 minutes. This gradient generator-based PRISM assay provides an integrated AST device that is viable for convenient point-of-care testing and offers a promising and most importantly, rapid alternative to current clinical practices, which extend to 8-24 h.",
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N1 - Funding Information: This research was funded by the German Research Foundation (DFG) via the Emmy Noether program (project ID 346772917), by the VolkswagenStiftung via the program “Niedersächsisches Vorab: Research Cooperation Lower Saxony—Israel”, and by the DFG via the grant SCHE 279/32-2. We also wish to thank Dima Peselev and Orna Ternyak of the Technion's at the micro- and nano-fabrication and printing unit for the microfabrication of the photonic silicon chips. Part of the cartoons in Fig. 1 and 4 were obtained from https://BioRender.com under a publishing license. The open access publication of this article was supported by the DFG sponsored Open Access Fund of the University of Augsburg.

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