TY - JOUR

T1 - 3D-printed flow cells for aptamer-based impedimetric detection of E. coli crooks strain

AU - Siller, Ina G.

AU - Preuss, John Alexander

AU - Urmann, Katharina

AU - Hoffmann, Michael R.

AU - Scheper, Thomas

AU - Bahnemann, Janina

N1 - Funding Information: This research was funded by the Bill and Melinda Gates Foundation (Grant Number OPP1111252) and the German Research Foundation (DFG) via the Emmy Noether program (project ID 346772917). The publication of this article was funded by the Open Access fund of Leibniz Universit?t Hannover. The authors would like to thank the Graduate Academy of the Leibniz University of Hannover for supporting the collaboration and work with the California Institute of Technology in Pasadena,.

PY - 2020/8/7

Y1 - 2020/8/7

N2 - Electrochemical spectroscopy enables rapid, sensitive, and label‐free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen‐printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio‐)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high‐resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor‐made, experimentspecific systems. In this work, flow cells for SPE‐based electrochemical (bio‐)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamerbased impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D‐printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high‐resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio‐)sensor applications.

AB - Electrochemical spectroscopy enables rapid, sensitive, and label‐free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen‐printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio‐)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high‐resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor‐made, experimentspecific systems. In this work, flow cells for SPE‐based electrochemical (bio‐)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamerbased impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D‐printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high‐resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio‐)sensor applications.

KW - Additive manufacturing

KW - Aptasensor

KW - Impedimetric biosensor

KW - Screen‐printed electrodes

UR - http://www.scopus.com/inward/record.url?scp=85089225148&partnerID=8YFLogxK

U2 - 10.3390/s20164421

DO - 10.3390/s20164421

M3 - Article

C2 - 32784793

AN - SCOPUS:85089225148

VL - 20

JO - Sensors

JF - Sensors

SN - 1424-3210

IS - 16

M1 - 4421

ER -