3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autorschaft

  • P. S. Timashev
  • M. V. Vedunova
  • D. Guseva
  • E. Ponimaskin
  • A. Deiwick
  • T. A. Mishchenko
  • E. V. Mitroshina
  • A. V. Koroleva
  • A. S. Pimashkin
  • I. V. Mukhina
  • V. Ya Panchenko
  • B. N. Chichkov
  • V. N. Bagratashvili

Externe Organisationen

  • Russian Academy of Sciences (RAS)
  • Nizhni Novgorod State University
  • Medizinische Hochschule Hannover (MHH)
  • Laser Zentrum Hannover e.V. (LZH)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer035001
FachzeitschriftBiomedical Physics and Engineering Express
Jahrgang2
Ausgabenummer3
PublikationsstatusVeröffentlicht - 27 Apr. 2016
Extern publiziertJa

Abstract

Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against βIII-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic-inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.

ASJC Scopus Sachgebiete

Zitieren

3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function. / Timashev, P. S.; Vedunova, M. V.; Guseva, D. et al.
in: Biomedical Physics and Engineering Express, Jahrgang 2, Nr. 3, 035001, 27.04.2016.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Timashev, PS, Vedunova, MV, Guseva, D, Ponimaskin, E, Deiwick, A, Mishchenko, TA, Mitroshina, EV, Koroleva, AV, Pimashkin, AS, Mukhina, IV, Panchenko, VY, Chichkov, BN & Bagratashvili, VN 2016, '3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function', Biomedical Physics and Engineering Express, Jg. 2, Nr. 3, 035001. https://doi.org/10.1088/2057-1976/2/3/035001
Timashev, P. S., Vedunova, M. V., Guseva, D., Ponimaskin, E., Deiwick, A., Mishchenko, T. A., Mitroshina, E. V., Koroleva, A. V., Pimashkin, A. S., Mukhina, I. V., Panchenko, V. Y., Chichkov, B. N., & Bagratashvili, V. N. (2016). 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function. Biomedical Physics and Engineering Express, 2(3), Artikel 035001. https://doi.org/10.1088/2057-1976/2/3/035001
Timashev PS, Vedunova MV, Guseva D, Ponimaskin E, Deiwick A, Mishchenko TA et al. 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function. Biomedical Physics and Engineering Express. 2016 Apr 27;2(3):035001. doi: 10.1088/2057-1976/2/3/035001
Timashev, P. S. ; Vedunova, M. V. ; Guseva, D. et al. / 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function. in: Biomedical Physics and Engineering Express. 2016 ; Jahrgang 2, Nr. 3.
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T1 - 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function

AU - Timashev, P. S.

AU - Vedunova, M. V.

AU - Guseva, D.

AU - Ponimaskin, E.

AU - Deiwick, A.

AU - Mishchenko, T. A.

AU - Mitroshina, E. V.

AU - Koroleva, A. V.

AU - Pimashkin, A. S.

AU - Mukhina, I. V.

AU - Panchenko, V. Ya

AU - Chichkov, B. N.

AU - Bagratashvili, V. N.

PY - 2016/4/27

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AB - Zr-Si organic-inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against βIII-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic-inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.

KW - 3D neural cell culture

KW - Ca imaging

KW - Dissociated hippocampal cultures

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