3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Vitalii V. Mutsenko
  • Vasilii V. Bazhenov
  • Olena Rogulska
  • Dmitriy N. Tarusin
  • Kathleen Schütz
  • Sophie Brüggemeier
  • Elke Gossla
  • Ashwini R. Akkineni
  • Heike Meißner
  • Anja Lode
  • Stephan Meschke
  • Andre Ehrlich
  • Slavica Petović
  • Rajko Martinović
  • Mirko Djurović
  • Allison L. Stelling
  • Sergey Nikulin
  • Sergey Rodin
  • Alexander Tonevitsky
  • Michael Gelinsky
  • Alexander Y. Petrenko
  • Birgit Glasmacher
  • Hermann Ehrlich

Organisationseinheiten

Externe Organisationen

  • Technische Universität Bergakademie Freiberg
  • Technische Universität Dresden
  • BromMarin GmbH
  • University of Montenegro
  • Duke University
  • Moscow Institute of Physics and Technology
  • P. Hertsen Moscow Oncology Research Institute
  • Nationale Akademie der Wissenschaften der Ukraine
  • Institute for Problems of Cryobiology and Cryomedicine
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)1966-1974
Seitenumfang9
FachzeitschriftInternational Journal of Biological Macromolecules
Jahrgang104
Frühes Online-Datum25 März 2017
PublikationsstatusVeröffentlicht - Nov. 2017

Abstract

The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.

ASJC Scopus Sachgebiete

Ziele für nachhaltige Entwicklung

Zitieren

3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells. / Mutsenko, Vitalii V.; Bazhenov, Vasilii V.; Rogulska, Olena et al.
in: International Journal of Biological Macromolecules, Jahrgang 104, 11.2017, S. 1966-1974.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Mutsenko, VV, Bazhenov, VV, Rogulska, O, Tarusin, DN, Schütz, K, Brüggemeier, S, Gossla, E, Akkineni, AR, Meißner, H, Lode, A, Meschke, S, Ehrlich, A, Petović, S, Martinović, R, Djurović, M, Stelling, AL, Nikulin, S, Rodin, S, Tonevitsky, A, Gelinsky, M, Petrenko, AY, Glasmacher, B & Ehrlich, H 2017, '3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells', International Journal of Biological Macromolecules, Jg. 104, S. 1966-1974. https://doi.org/10.1016/j.ijbiomac.2017.03.116
Mutsenko, V. V., Bazhenov, V. V., Rogulska, O., Tarusin, D. N., Schütz, K., Brüggemeier, S., Gossla, E., Akkineni, A. R., Meißner, H., Lode, A., Meschke, S., Ehrlich, A., Petović, S., Martinović, R., Djurović, M., Stelling, A. L., Nikulin, S., Rodin, S., Tonevitsky, A., ... Ehrlich, H. (2017). 3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells. International Journal of Biological Macromolecules, 104, 1966-1974. https://doi.org/10.1016/j.ijbiomac.2017.03.116
Mutsenko VV, Bazhenov VV, Rogulska O, Tarusin DN, Schütz K, Brüggemeier S et al. 3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells. International Journal of Biological Macromolecules. 2017 Nov;104:1966-1974. Epub 2017 Mär 25. doi: 10.1016/j.ijbiomac.2017.03.116
Download
@article{6e6fb676313a417687ab78ea1e4df922,
title = "3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells",
abstract = "The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.",
keywords = "Chitin scaffolds, Mesenchymal stromal cells, Sponges",
author = "Mutsenko, {Vitalii V.} and Bazhenov, {Vasilii V.} and Olena Rogulska and Tarusin, {Dmitriy N.} and Kathleen Sch{\"u}tz and Sophie Br{\"u}ggemeier and Elke Gossla and Akkineni, {Ashwini R.} and Heike Mei{\ss}ner and Anja Lode and Stephan Meschke and Andre Ehrlich and Slavica Petovi{\'c} and Rajko Martinovi{\'c} and Mirko Djurovi{\'c} and Stelling, {Allison L.} and Sergey Nikulin and Sergey Rodin and Alexander Tonevitsky and Michael Gelinsky and Petrenko, {Alexander Y.} and Birgit Glasmacher and Hermann Ehrlich",
note = "Funding information: The present work was partially supported by Leonhard-Euler-Programm from German Academic Exchange Service (DAAD, Germany) as well as DFG Grant EH 394-3 (DFG, Germany). The authors would like to thank Igor Kovalenko, Galina Bozhok, Vitaliy Kholodny and Ortrud Zieschang for excellent technical assistance.",
year = "2017",
month = nov,
doi = "10.1016/j.ijbiomac.2017.03.116",
language = "English",
volume = "104",
pages = "1966--1974",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",

}

Download

TY - JOUR

T1 - 3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells

AU - Mutsenko, Vitalii V.

AU - Bazhenov, Vasilii V.

AU - Rogulska, Olena

AU - Tarusin, Dmitriy N.

AU - Schütz, Kathleen

AU - Brüggemeier, Sophie

AU - Gossla, Elke

AU - Akkineni, Ashwini R.

AU - Meißner, Heike

AU - Lode, Anja

AU - Meschke, Stephan

AU - Ehrlich, Andre

AU - Petović, Slavica

AU - Martinović, Rajko

AU - Djurović, Mirko

AU - Stelling, Allison L.

AU - Nikulin, Sergey

AU - Rodin, Sergey

AU - Tonevitsky, Alexander

AU - Gelinsky, Michael

AU - Petrenko, Alexander Y.

AU - Glasmacher, Birgit

AU - Ehrlich, Hermann

N1 - Funding information: The present work was partially supported by Leonhard-Euler-Programm from German Academic Exchange Service (DAAD, Germany) as well as DFG Grant EH 394-3 (DFG, Germany). The authors would like to thank Igor Kovalenko, Galina Bozhok, Vitaliy Kholodny and Ortrud Zieschang for excellent technical assistance.

PY - 2017/11

Y1 - 2017/11

N2 - The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.

AB - The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7 days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.

KW - Chitin scaffolds

KW - Mesenchymal stromal cells

KW - Sponges

UR - http://www.scopus.com/inward/record.url?scp=85016395031&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2017.03.116

DO - 10.1016/j.ijbiomac.2017.03.116

M3 - Article

C2 - 28347785

AN - SCOPUS:85016395031

VL - 104

SP - 1966

EP - 1974

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -